Figure 3. Migration analysis of NTF4 proteins. Scratch wounds were created with 200-μl pipette tips on the NTF4-transfected HeLa cells. The medium was replaced by fresh serum-free medium. Photomicrographs were taken at time 0, 24, and 48 h. The wound gaps were measured, and the percent migration was calculated by the average area reduction of 24 or 48 h as compared with time 0. The average area reduction in HeLa cells expressing NTF4 wild type protein was larger than that in cells expressing NTF4 variants at hour 24 and 48.