Figure 2. The effect of Y-27632 on
the transformation of keratocytes. A: On
immunohistochemistry α-SMA expression was seen in about 4% of
cells in culture with TGFβ1 stimulation, but in only 0.3% of
cells treated with TGFβ1 and Y-27632 (p<0.01, Student’s t-test).
Values are means±SEM (n=6). Scale bar: 200 μm. B:
Western blots revealed that the expression of α-SMA was
significantly decreased in keratocytes treated with Y-27632, but
not abolished.