Figure 2. The effect of Y-27632 on the transformation of keratocytes. A: On immunohistochemistry α-SMA expression was seen in about 4% of cells in culture with TGFβ1 stimulation, but in only 0.3% of cells treated with TGFβ1 and Y-27632 (p<0.01, Student’s t-test). Values are means±SEM (n=6). Scale bar: 200 μm. B: Western blots revealed that the expression of α-SMA was significantly decreased in keratocytes treated with Y-27632, but not abolished.