Figure 6. Effects of heparanase expression levels on human retinal vascular endothelial cell AKT and extracellular signal regulated kinase (ERK) protein expression western blotting analyses showed that the levels of phosphorylated ERK and Akt were enhanced by heparanase overexpression. A, B: HPSE-LV-Glu30 human retinal vascular endothelial cells (HRECs) showed significantly reduced ERK and Akt phosphorylation levels compared with the Con-LV-Glu30 and Glu30 groups. The ERK and Akt phosphorylation levels of the Glu30 HRECs were significantly higher than the HPSE-LV-Glu30 and Glu5 groups. (●p<0.01 versus Glu5 p-Akt; ▲p<0.01 versus HPSE-LV-Glu30 p-Akt; ★p<0.01 versus Glu5 p-ERK; ■p<0.01 versus HPSE-LV-Glu30 p-ERK). Bars indicate the mean±SD, p<0.05. Each group experiment was repeated 3 times (n=3).