Figure 3. Expression of heparanase protein and mRNA in high-glucose-treated human retinal vascular endothelial cells is suppressed by RNAi. A, B: HPSE-LV significantly inhibited human retinal vascular endothelial cells (HRECs) heparanase protein expression in the heparanase small interfering RNA recombinant lentiviral vector (HPSE-LV)-Glu30 group compared with HRECs in the Glu30 and Con-LV-Glu30 groups (●p<0.01 versus Glu5; ▲p<0.01 versus HPSE-LV-Glu30 group). Each group experiment was repeated 3 times (n=3). C: Real-time PCR analysis showed that heparanase mRNA was markedly inhibited in the HPSE-LV-Glu30 group compared with HRECs in the Glu30 and Con-LV-Glu30 groups (●p<0.01 versus Glu5; ▲p<0.01 versus HPSE-LV-Glu30). Each group experiment was repeated 3 times (n=3).