Figure 2. Expression of heparanase in
human retinal vascular endothelial cells is suppressed by RNAi.
A: Human retinal vascular endothelial cells (HRECs) were
infected with Con-LV or heparanase small interfering RNA
recombinant lentiviral vector (HPSE-LV) at a multiplicity of
infection (MOI) of 60. Green fluorescent protein (GFP)
expression and phase contrast images were captured after 72 h
(original magnification × 200). B: The heparanase mRNA
levels were detected using real-time PCR after the HRECs were
treated with HPSE-LV and Con-LV. HPSE-LV significantly inhibited
the expression of HREC heparanase mRNA (●p<0.01, compared
with HRECs; ▲p<0.01, compared with the Con-LV group). Each
group experiment was repeated 3 times (n=3). C: western
blotting analysis showed that the heparanase protein was
markedly inhibited by HPSE-LV in HRECs.