Figure 6. Histopathology of RGC loss.
Light microscopy of the eyes of mice euthanized a year post AAV
injections confirmed the OCT findings of inner retinal loss
induced by injection of the mutant ND4 MTS AAV showing
marked focal thinning of the inner retina at low (A) and
higher (B) magnifications. C: While experimental
eyes of other animals did not exhibit such severe inner retinal
atrophy, the more characteristic loss of cells in the ganglion
cell layer was evident. Control eyes injected with scAAV-GFP
showed no loss of the inner retina layers (D and E)
or cells in the RGC layer (F). ON=optic nerve,
RGC=retinal ganglion cell layer, INL=inner nuclear layer, and
ONL=outer nuclear layer.