Figure 4. Mutant human ND4 expression induces visual loss in mice. A: At 1 month post injection, a scatterplot of a root-mean-square (RMS) PERG amplitude shows a significant loss of visual function of the right eyes injected with the mutant human ND4 packaged with the MTS scAAV relative to the left eyes injected with GFP packaged with standard scAAV (p=0.0352). B: Six months post injection, this decrement between experimental and control eyes was even more significant (p=0.0182). C: Representative waveforms show normal amplitude 6 months after scAAV-GFP injection but loss (D) of PERG signal with MTS scAAV mutant ND4 injection. E: A barplot shows an increasing reduction of the ratio of the right eye to left eye amplitudes with 1-month (1M) and 6-month (6M) intervals post injection relative to baseline values (BL) obtained before AAV injections.