Table 1 of Kucerova, Mol Vis 2012; 18:139-150.
Gene | Forward Sequence 5′-3′ | Reverse Sequence 5′-3′ | Product Size (bp) | Annealing temp (°C) |
---|---|---|---|---|
Shh | CCCTTTAGCCTACAAGCAGT | CCACTGGTTCATCACAGAG | 232 | 57 |
Dhh | ACATCACCACGTCTGACC | GAACACCGGTGCAAAGTCAC | 309 | 58 |
Ihh | ACGTGCATTGCTCTGTCAAGT | CTGGAAAGCTCTCAGCCGGTT | 221 | 58 |
Ptch1 | CTGCTGCTATCCATCAGCGT | AAGAAGGATAAGAGGACAGG | 452 | 58 |
Ptch2 | TGCCTCTCTGGAGGGCTTCC | CAGTTCCTCCTGCCAGTGCA | 207 | 58 |
Smo | GTGATGATGAGCCCAAGAGA | AGGGGCAGAGTGGTGAAGC | 422 | 58 |
Gli1 | CAGGGAAGAGAGCAGACTGA | AGCTGATGCAGCTGATCCAG | 251 | 60 |
Gli2 | ACGACTTTCTCCACACCCTGC | TGATGTAAGCTACCAGCGAG | 478 | 60 |
Gli3 | ATCCGCTCATTGCACAGCAG | TGAAGCTCAATGCAGGGCTG | 436 | 58 |
Hprt | CCTGTCGGATTACATTAAAGCACTG | GTCAAGGGCATATCCAACAACAAC | 301 | 57 |
Gsk3β | CAG CCT TCA GCT TTT GGT AGC | TCCACCAACTGATCCACACCAC | 750 | 58 |
Pax6 | AGTTCTTCGCAACCTGGCTA | TGAAGCTGCTGCTGATAGGA | 519 | 62.5 |
Su(Fu) | GCT CCA GGT TAC CGC TAT CG | GCT GCC CAA ACT GTC CTTGC | 772 | 58 |