Figure 6. Gli3 localization in wild-type and Pax6^+/− corneal epithelia. A-D: Localization of Gli3 protein in adult Pax6^+/+ (WT; A, C) and Pax6^+/− (+/−; B, D) corneal epithelia using antibodies directed against the activator COOH-terminal fragment and the repressor NH₂-terminal form of the protein. Nuclear COOH-terminal protein is present in wild-type epithelial nuclei but is not easily detectable in Pax6^+/− corneas. E: The negative control is with no primary antibody. Scale bar: 50 μm.