Figure 2. H2O2 dose-dependently decreased cell viability of retinal pigment epithelial cells as tested by microculture tetrazoline test.
Retinal pigment epithelial (RPE) cells were plated in 96-well plates and treated with various concentrations of H2O2 for 24 h. Cell viability was evaluated by the microculture tetrazoline test (MTT) test and expressed as percentage of that
in cells without H2O2 (mean±standard deviation [SD] in triplicate tests). A: H2O2 showed dose-dependent cytotoxic effects on ARPE-19 cells (cells from an immortal RPE cell line from a 19-year-old donor)
at concentrations from 0.25 mM to 1.00 mM (p<0.05, compared to cells cultured without H2O2. B: Studies of primary cultures of human RPE cells isolated from donor eye showed similar results. *p<0.05, compared with the
controls (cells cultured with H2O2 alone).