Molecular Vision: Varma, Mol Vis 2012; 18:1609-1618. Table 2

Table 2. List of miRNAs up- or downregulated in galactose + pyruvate-stimulated condition in mice.

miRNAs	Gal + Pyru (G2 group) (2^(-∆Ct))	Normal (control group) (2^(-∆Ct))	Fold regulation
Mmu-miR-16	19.73494	4.494815	4.3906
Mmu-miR-21	9.972084	1.787833	5.5778
Mmu-miR-126–3p	1.536338	0.337068	4.5579
Mmu-miR-322	0.723999	0.140242	5.1625
Mmu-miR-872	2.242205	0.51421	4.3605
Mmu-miR-126–5p	0.983431	0.155496	6.3245
Mmu-miR-196b	0.000403	0.00005	8.0097
Mmu-miR-150	0.061455	0.014682	4.1857
Mmu-miR-196a	0.001338	0.0003	4.4645
Mmu-miR-1	68.42511	14.05485	4.8684
Mmu-miR-335–5p	0.286922	0.032472	8.836
Mmu-miR-374	3.971576	0.845519	4.6972
Mmu-miR-142–5p	0.000164	0.0012	-7.3161
Mmu-miR-142–3p	0.001725	0.015434	-8.9486
Mmu-miR-29b	0.008598	0.037339	-4.3427
Mmu-miR-32	0.038068	0.206379	-5.4213
mumiR-144	0.000096	0.001222	-12.7342
Mmu-miR-214	0.000418	0.001989	-4.7579

The table represents the effect of pyruvate on miRNA transcription in the lenses of galactosemic mice. Numbers in italics represent upregulation of the genes considered to be primarily apoptotic, except for miR-21, which can reportedly act as anti-apoptotic as well as pro-apoptotic. miRNAs in bold are downregulated. Values under the column titled Fold Regulation represent normalized gene expression (2^(- ∆Ct)) in the galactose + pyruvate (G2 group) divided by the normalized gene expression (2^(- ∆Ct)) in the normal (control) group. The raw Cts were normalized to housekeeping genes, reagent controls, and PCR efficiency. It is to be noted that the relative upregulation of the genes in the gal+pyruvate group/normal controls in this case is mostly lower than that caused in the galactose-alone group, as mentioned in Table 1.