Figure 5. There was no significant increase of apoptotic RGC-5 cells after treatment with YC-1. A: RGC-5 cells were treated with 20 µM YC-1 or 0.066% DMSO for 24 h. Apoptosis of cells was assessed using annexin V/propidium iodide staining and flow cytometry. B: The histogram shows the representative flow cytometry data of three experiments.