Figure 4. YC-1 had no influence on RGC-5 cell death. A: After treatment with 20 µM YC-1 or 0.066% DMSO for 24 h, RGC-5 cells were stained with calcein AM (green for live cells), EthD-1 (red for dead cells), and Hoechst 33342 (blue for nuclei) for 40 min at room temperature. Representative photomicrographs show the cell density and composition. B: Cell viability was examined by calcein AM/EthD-1 fluorescence staining and flow cytometry.