Figure 3. An increase of the YC-1 exposure time resulted in a decrease of the RGC-5 cell density. A: RGC-5 cells were exposed to 20 µM YC-1 for 4, 12, and 24 h. Morphological changes in cell density were observed with light microscopy. B: Cell viability was detected by an MTT assay of RGC-5 cells treated with YC-1 for different incubation times. * Indicates p<0.05 compared to the DMSO vehicle group.