Figure 3. Expression of ANXA1 in the retinal pigment epithelium (RPE) by ultrastructural analysis. Control group (A-C). Experimental groups: 24 h (D and E), 48 h (F), and 72 h (G). A: Electron micrograph of RPE localized between a vessel (V) of the choriocapillaris and light-sensitive outer segments of the photoreceptors (P). Marked vacuolization of the RPE (D) is observed after intravitreal inoculation of T. gondii compared to the control group (B). ANXA1 immunogold labeling (arrows) in the cytosol, vacuoles and membrane of pigment granules of RPE of control (C) and infected eyes after 24 h (E). Also, a significant proportion of ANXA1 immunoreactivity (arrows) observed in infected eyes after 48 (F) and 72 h (G). No labeling was detected in the control section (H). Scale bars: 1 µm (A and D), 0.5 µm (B, C, E, F, G, and H). Density of ANXA1 immunogold particles in RPE (I). Data are mean±SEM of 10 distinct cells analyzed from four mice per group. **p<0.01 and ***p<0.001 versus control group.