Table 1 of
Banerjee, Mol Vis 2012; 18:1548-1557. Table 1 of
Banerjee, Mol Vis 2012; 18:1548-1557.
Table 1. Primers used for PCR and sequencing of the MYOC coding region.
Primer ID |
Location |
Primer sequences |
Tm |
Ampilcon size (bp) |
|---|---|---|---|---|
| PCR primers | ||||
| TIGR-1F | Exon 1 | 5'- GGCTGGCTCCCCAGTATATA -3' | 62 | 762 |
| TIGR-6R | 5'- CTGCTGAACTCAGAGTCCCC -3' | 64 | ||
| TIGR-8F | Exon 3 | 5'- TTATGGATTAAGTGGTGCTTCG -3' | 54 | 860 |
| TIGR-13R | 5'- AGCATCTCCTTCTGCCATTG -3' | 60 | ||
| Sequencing primers | ||||
| TIGR-3F | Exon 1 | 5'-AGTGGCCGATGCCAGTATAC-3' | 62 | |
| TIGR-3R | 5'-CTGGTCCAAGGTCAATTGGT-3' | 60 | ||
| TIGR-10F | Exon 3 | 5'- ATACTGCCTAGGCCACTGGA-3' | 62 | |
| TIGR-10R | 5'- CAATGTCCGTGTAGCCACC -3' | 62 | ||