Figure 5. Electron micrographs of
electron dense filaments stained with Cuprolinic blue on stromal
collagen. Samples of A: WT and B: P2X7−/−
corneas were digested with either Keratanase I, Chondroitinase
ABC, or both, before Cuprolinic blue staining and imaging with
TEM. Undigested and stained tissue are included. The scale bar
seen in the undigested column represents 500 nm, and applies to
all images in the Undigested, Keratanase I, and Chondroitinase
ABC columns. The scale bar seen in the Keratanase I and
Chondroitinase ABC columns represent 200 nm and applies to
images in that column only. Descemet’s membrane is marked with
“D” and is visible in the WT posterior images.