Figure 4. Decorin localization is altered in P2X₇^−/− stromas. Frozen corneas were sectioned and stained with antibody against decorin, FITC-conjugated IgG, and To-Pro 3AM. Negative controls were incubated with non-immune IgG instead of primary antibody, and To-Pro 3AM. Images are representative of three independent experiments. E=Epithelium, S=Stroma. Scale bar: 50 μm.