Figure 3. KSPG localization is altered in P2X₇^−/− stromas. Frozen corneas were sectioned and stained with antibodies specific for keratocan and lumican, followed by FITC-conjugated IgG (green) and counterstained with To-Pro 3AM for nuclei (blue). Negative controls were incubated with secondary antibody only and counterstained with To-Pro 3AM. Images are representative of three independent experiments. Keratocan (inset): enlarged regions from central stroma with enhanced signal to show detail of localization. Scale bar: 10 μm.