Figure 5. Effect of 9-cis-retinal on M-opsin expression in Rpe65^−/− mice in retinal explants. A: Retina-RPE choroids incubated with 0.5 nM or 5 nM 9-cis-retinal for 24 h were used for western blotting to detect M-opsin protein. B: Immunolocalization of M-opsin treated 0.5 nM 9-cis-retinal for 24 h in the Rpe65^−/− mice retina. Immunoreactivity of M-opsin (red) was higher with 0.5 nM 9-cis-retinal treatment compared to vehicle treatment in the cone outer segments (arrows), ONL, and OPL (arrowheads) in the retina. C: Histological findings of the density on the reactions labeled anti-M-opsin antibody in a 400-μm-wide section of the retina. Values represented mean ± SD (n=6). ***p<0.001. RPE, retinal pigment epithelium; OS, outer segment; ONL, outer nuclear layer; OPL, outer plexiform layer. Scale bars, 50 μm.