Figure 2. Detection of apoptotic cells with TUNEL staining. Wild-type retina in vivo as negative control (A), explant culture of the wild-type retina for 12 h (B), and 24 h incubation (C). Blue: nuclear staining with DAPI. Green: TUNEL-positive staining indicated by arrows. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bars=100 μm.