Figure 7. In vitro assay of attachment of human corneal epithelial cells (HCECs). The effect of anti-SCF antibodies, tyrosine kinase
inhibitor genistein, RGD peptide, and extracellular matrix on the attachment of HCECs was examined. The HCECs were incubated
with 0, 10, 50, 250, or 1,000 ng/ml of anti-SCF antibodies (A), 0, 5, 10, 25, or 50 µg/ml of genistein (B), or 0, 125, 250, 500, or 1,000 µg/ml of GRGDSP or GRGESP peptides (C). The HCECs were seeded on fibronectin, laminin, or type IV collagen-coated dishes incubated with 1,000 ng/ml of anti-SCF
antibodies (D). The results are normalized to the number of controls. Error bars represent the standard errors (n=3). *p<0.01, +p<0.05 (Man-Whitney U test) against controls.