Figure 8. Changes in protein expression were identified using immunocytochemical analysis. Expression of seven selected markers were confirmed as proteins. Markers examined included Sox2 (A), K_i-67 (B), β3-tubulin (C), Map2 (D), glial markers glial fibrillary acidic protein (GFAP; E), Krüppel-like factor 4 (KLF4; F), and C-X-C chemokine receptor type 4 (CXCR4; G). Insets provide higher power images of labeled profiles to illustrate labeling patterns. Photomicrographs showing labeling for each marker under standard medium (SM), ciliary neurotrophic factor (CNTF), and fetal bovine serum (FBS) conditions from left to right, respectively. Insets in F and G provide higher power images of labeled profiles, to illustrate labeling patterns. In addition, results after 24 h treatment were compared across treatment conditions as shown in the accompanying histograms. In each case, untreated cultures in SM are represented by the left bar, the CNTF-based condition by the center bar, and the FBS-based condition by the right bar. Error bars show standard deviation (SD); asterisks (*) denote statistical significance (p<0.05). Scale bar: 100 µm.