Figure 7. Examination of 15 selected gene expression profiles using polymerase chain reaction (PCR) shows significant changes with treatment and time. Progenitor markers nestin (A), Sox2 (B), Vimentin (C), K_i-67 (D), Krüppel-like factor 4 (Klf4; J), c-myc (K), and C-X-C chemokine receptor type 4 (CXCR4; L) are profiled across time for each treatment. Mature neural markers β3-tubulin (E) and Map2 (F) as well as mature glial markers glial fibrillary acidic protein (GFAP; G), myelin basic protein (MBP; H), and Claudin 11 (I) are profiled as well. Changes to gene expression relating to the Jak-STAT signaling pathway, Janus kinase 1 (Jak1; M), signal transducer and activator of transcription 3 (STAT1; N) and signal transducer and activator of transcription 3 (STAT3; O), are also shown. Fold changes for the 15 gene profiles were normalized to standard medium (SM) day 0. Data from treatment day 1 are shown as red bars, day 3 as green bars, and day 5 as blue bars. Both fetal bovine serum (FBS) and ciliary neurotrophic factor (CNTF) treated groups are compared to SM, respective to time point, and then significance was established using the Student’s t-test. Error bars represent standard error of the mean (SEM). *=p<0.05. β-Actin was used as the endogenous control.