Figure 6. Genes were examined during the time course of treatment using real-time polymerase chain reaction (PCR). Fifteen genes were profiled across five days following treatment with either standard medium (SM, A), ciliary neurotrophic factor (CNTF; B), or fetal bovine serum (FBS; C). Fold changes in expression level were calculated with RQ using the ΔΔCt method, normalized to SM day 0. Samples were run in triplicate and both CNTF and FBS conditions were compared to the respective SM data for days 1, 3 and 5, with significance established using the Student’s t-test. Error bars represent standard error of the mean (SEM). *=p<0.05.