Figure 5. Expression patterns of selected genes were confirmed using quantitative real-time polymerase chain reaction (PCR). Confirmation of selected immature and lineage-related genes was undertaken using quantitative real-time PCR and displayed as a global view heat map across treatment conditions and time points in culture, the latter indicated along the abscissa. As indicated by the group labels, all porcine neural progenitor cultures were originally derived from brain tissue at embryonic day 45 (E45-pBPC). Untreated controls (standard medium [SM]) have titles in green text, ciliary neurotrophic factor (CNTF) treatment in red, and fetal bovine serum (FBS) in blue. The results were further subjected to cluster analysis employing Pearson’s correlation coefficient as a distance measure, as indicated by dendrograms at top and left. For the heat map scale (right), black is set as the median of all ΔCT values (23) obtained in the study, while increasing intensity of red represents higher gene expression levels and increasing green shows lower levels.