Figure 7. Effect of palmitate on p38, ERK, and JNK phosphorylation in orbital fibroblasts. Orbital fibroblasts were treated with palmitate
(200 μM) for 0.5– 24 h (
A) or treated with palmitate (50–200 μM) for 24 h (
B). Levels of phosphorylated p38, ERK, and JNK were determined by immunoblot analysis. Data are representative of three independent
experiments using orbital fibroblasts from #1, #3, and #4 patients with TAO (
Table 1).