Figure 3. Secretion of IL-6, IL-8, MCP-1, and hyaluronic acid (HA) from orbital fibroblasts in response to IL-1β. Orbital fat-derived fibroblasts were treated with IL-1β (10 ng/ml) for 24 h. Concentrations of IL-6 (A), IL-8 (B), MCP-1 (C), and HA (D) were determined by ELISA. Data are presented as the mean±SD of three replicate culture wells from a representative experiment. Similar results were observed in orbital fibroblasts from #1 and #2 patients with TAO (Table 1). * p<0.05.