Figure 6. Established hypertension alone or in combination with diabetes stimulates retinal inflammation. A: Representative image for western blot analysis of retinal nuclear factor kappaB p65 (NFkB p65) protein expression in the established stage of spontaneous hypertensive rats (SHR) and diabetic spontaneous hypertensive rats (SHR+D) compared to control wistar group (W). B: Representative image for western blot analysis of retinal tumor necrosis factor aplha (TNF-α) protein expression in the established stage of SHR and SHR+D compared to control wistar group (W; n=4, *p<0.05). C: Representative image shows results of western blot analysis of retinal TNF-α protein expression. D: Statistical analysis showed that TNF-α protein expression was higher in the SHR and SHR+D groups by 3.4 and 5.4 fold, respectively, relative to the control W group (n=4, *p<0.05).