Figure 1. Gene expression data analysis. A PCA plot of all samples was generated using Partek Genomic Suite 6.5 beta to assess the variability of microarray data (Color for scan date and shapes for time points). It was shown that the individual scleral samples from each time point were more related to each other than samples from other time points, demonstrating that there were significant differences in the gene expressions between the various age groups (A). Furthermore, the mean of all time points was hierarchically clustered, and it was demonstrated they did not cluster according to age (B).