Figure 3. Expression levels of FRMD7 at the indicated time points in Neuro-2a cells were confirmed by western blot. FRMD7 was monitored by western blot in transfected Neuro-2a cells at the indicated time points. Samples (cells) from cultured Neuro-2a cells transfected with pcDNA3.1 (+)-FRMD7-flag at different time points (24 h, 48 h, 72 h, and 7 days) were detected. The blots were probed with the labeled antibody anti-flag. β-actin was used as an internal control.