Figure 2. Overexpression of FRMD7 regulated the mRNA expression levels of neuron-specific genes in Neuro-2a cells. Cells transfected with the empty vector served as the negative control (MOCK group). The expression of Mtap2, NF-L, NF-M, and NeuN mRNA (mRNA) doubled 48 h after transfection. The expression levels of NF-L and NF-M mRNA continued to increase, reaching a 2.2-fold increase 7 days after transfection. The mRNA levels of other genes (Nestin, NF-H, MAPT, GAP-43, and Tuj-1) did not change obviously. The data are presented as changes relative to the MOCK group. All of the experiments were performed in triplicate, and the graph represents the average (columns, mean; bars, SEM; *p<0.05, **p<0.01 versus MOCK group).