Figure 1. Effects of FRMD7 overexpression on neurite outgrowth in undifferentiated or differentiated Neuro-2a cells. A, B: Neuro-2a cells transfected with FRMD7-pEGFP-n1 (B) or the empty pEGFP-n1 vector (A) was treated with retinoic acid (RA) for 3 days. The cells were fixed and stained with TRITC-conjugated rhodamine-phalloidin. Statistics were gathered on the percentage of cells that bore neurites and the length of the longest outgrowing neurite from the cell bodies of the evaluated cells. FRMD7 demonstrated a remarkable potential to increase neurite length compared with control cells. However, the number of cells with neurites was no different between groups. The overexpression of FRMD7 without RA treatment did not change the morphology of Neuro-2a cells (data not shown). Magnification: 100x. Scale bars: 50 μm. C: The average length of the longest outgrowing neurite from differentiated Neuro-2a cells transfected with FRMD7-pEGFP-n1 (FRMD7) or the empty pEGFP-n1 vector (CON). **p<0.01 compared with the control.