Table 1 of
Abu-Amero, Mol Vis 2012; 18:1421-1427. Table 1 of
Abu-Amero, Mol Vis 2012; 18:1421-1427.
Table 1. Primer sequences, PCR annealing temperature and amplicon size for OPTN.
Exon |
Primer sequence |
Annealing temp. (°C) |
Amplicon size (bp) |
|---|---|---|---|
| OPTN-1F | TGTAAAACGACGGCCAGTCTTGGTCGGGTGGGGTAT | 57 | 420 |
| OPTN-1R | CAGGAAACAGCTATGACCGCGGGTACCGTTTTCAGG | 57 | |
| OPTN-2F | TGTAAAACGACGGCCAGTTCTTAGTCTTTTCAGTATGCATTGA | 59 | 351 |
| OPTN-2R | CAGGAAACAGCTATGACCAGTTTATTGTGTTTTTGGTTAAGAAGA | 59 | |
| OPTN-3F | TGTAAAACGACGGCCAGTTACACACACACGCACACACA | 57 | 253 |
| OPTN-3R | CAGGAAACAGCTATGACCTTCCTGTGGAAAAGTCACCTG | 57 | |
| OPTN-4aF | TGTAAAACGACGGCCAGTAAGTGGGCAACTTTTGGAGT | 57 | 391 |
| OPTN-4aR | CAGGAAACAGCTATGACCAAGGGAGCTCACCACCAAG | 57 | |
| OPTN-4bF | TGTAAAACGACGGCCAGTATCGCCAATGGGTTTGTG | 59 | 376 |
| OPTN-4bR | CAGGAAACAGCTATGACCAAGGGAGCTCACCACCAAG | 59 | |
| OPTN-5F | TGTAAAACGACGGCCAGTCAGAGCCATGTGGTCAAGTG | 57 | 402 |
| OPTN-5R | CAGGAAACAGCTATGACCCCATGAAAGGTTTTGATCTAGGA | 57 | |
| OPTN-6F | TGTAAAACGACGGCCAGTCCACTTCAGCCTCCCAGAG | 57 | 382 |
| OPTN-6R | CAGGAAACAGCTATGACCCTTGGCTTGTGTTGACAAGAA | 57 | |
| OPTN-7F | TGTAAAACGACGGCCAGTCAAAAATTCATCTTTTGTCTTTTTC | 57 | 272 |
| OPTN-7R | CAGGAAACAGCTATGACCACTTCCTCAGGTCACAACATTT | 57 | |
| OPTN-8F | TGTAAAACGACGGCCAGTAAGCAGTTCCTTTAAGCTGGTC | 59 | 352 |
| OPTN-8R | CAGGAAACAGCTATGACCCTTTAAATGGGTGAACTGTATGG | 59 | |
| OPTN-9F | TGTAAAACGACGGCCAGTCCCAATTGTAAACAATGTTCTTTTT | 57 | 302 |
| OPTN-9R | CAGGAAACAGCTATGACCAAAGCAAATAACCCATCACAAGA | 57 | |
| OPTN-10F | TGTAAAACGACGGCCAGTGGCTACTAATGGTTCAGCCTGT | 57 | 315 |
| OPTN-10R | CAGGAAACAGCTATGACCTGTAAAAATGTATATTTCAAAGGAGGA | 57 | |
| OPTN-11F | TGTAAAACGACGGCCAGTTTATATTGTACATAACCTTGGGGTTT | 59 | 349 |
| OPTN-11R | CAGGAAACAGCTATGACCCAAATCCGAATTCCAATCTGTATAA | 59 | |
| OPTN-12F | TGTAAAACGACGGCCAGTCTGGAGTGTTCAGAAGGTTGG | 57 | 293 |
| OPTN-12R | CAGGAAACAGCTATGACCCCAGATTTAGTGAAGGATTCATGT | 57 | |
| OPTN-13F | TGTAAAACGACGGCCAGTCTAAAACAGGCAGAATTATTTCAAAAC | 57 | 358 |
| OPTN-13R | CAGGAAACAGCTATGACCAGAAAATTACAAACATTCTAAACACCA | 57 | |
| OPTN-14F | TGTAAAACGACGGCCAGTGGCTATTGAAGGATACAGCACT | 55 | 330 |
| OPTN-14R | CAGGAAACAGCTATGACCTCAAATCAGGAACGTCTTTGG | 55 | |
| OPTN-15F | TGTAAAACGACGGCCAGTTTTTTCCCCTACTTCTGTGGAC | 59 | 279 |
| OPTN-15R | CAGGAAACAGCTATGACCGAATCCATTGTAGAGAATGAAGTGG | 59 | |
| OPTN-16aF | TGTAAAACGACGGCCAGTGAAGTTGAACTGATGTTAAAACTCG | 57 | 777 |
| OPTN-16aR | CAGGAAACAGCTATGACCTAAGGATTCTACTTTGCGAGTTGATG | 57 | |
| OPTN-16bF | TGTAAAACGACGGCCAGTGAGACGACACCACTGCACTC | 57 | 777 |
| OPTN-16bR | CAGGAAACAGCTATGACCAACAATGTAAAAGATTCATAAGCAAAA | 57 | |
| OPTN-16cF | TGTAAAACGACGGCCAGTCCCATTCACAGTGTAAAGAAGTT | 57 | 777 |
| OPTN-16cR | CAGGAAACAGCTATGACCCCTCCAGTATAAGATGATAAGGGAAA | 57 | |
| OPTNPROM-F | TGTAAAACGACGGCCAGTGCAGCTTCCCTCCTCCAC | 57 | 630 |
| OPTNPROM-R | CAGGAAACAGCTATGACCGGGGTGCCTAGGGCTGAT | 57 |