Figure 5. Quantification of Brn3a-labeled retinal ganglion cells in retinal flatmounts. A: Compared to control groups, diabetic mice have significantly decreased numbers of retinal ganglion cells (RGCs; n=10/group). B: There is a similar result of percentage of RGCs labeled with Brn3a. The percentage of RGCs for marker combination was calculated in relation to the number of RGCs. Data are expressed as means±standard deviation (SD; * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001).