Figure 3. Quantification of retinal ganglion cell loss in the ganglion cell layer. Cell loss was assessed by counting the cells with Brn3a (A) and NeuN (C) immunoreactivity. The number of retinal ganglion cells (RGCs) exhibits a significantly difference in diabetic and control mice (n=10/group). In the percentage of RGCs for Brn3a (B) and NeuN (D), both markers were calculated in relation to the number of cells counted. Data are expressed as means±standard deviation (SD; * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001).