Table 2 of
Blom, Mol Vis 2012; 18:1339-1353. Table 2 of
Blom, Mol Vis 2012; 18:1339-1353.
Table 2. Primer Sequences Used.
Gene |
Primer Sequences (5′-3′) |
PCR product (bp) |
Annealing temp (°C) |
|---|---|---|---|
| ADM | F: CGAAAGAAGTGGAATAAGTGGG | 200 | 60 |
| R: GTTCATGCTCTGGCGGTAGCG | |||
| CRLR | F: GCTGAATGACGTTGCAGC | 480 | 60 |
| R: GCCTTCACAGAGCATCCA | |||
| RAMP2 | F: CATGGACTCTGTCAAGGACTG | 153 | 55 |
| R: GTGTATCAGGTGAGCCT | |||
| RAMP3 | F: ACCTGTCGGAGTTCATCG | 262 | 55 |
| R: ATCAGTGTGCTTGCTGCG |