Figure 4. Immunocytochemical
localization of the adrenomedullin (ADM) receptor calcitonin
receptor like receptor (CRLR) and receptor activity modifying
protein (RAMP2). A: The ADM receptor CRLR-like
immunoreactivity (LI) was localized near the outer segments of
photoreceptors (diagonal arrow), in faint somata in the outer
nuclear layer (ONL; horizontal arrow), in puncta in the outer
plexiform layer (OPL; asterisk), in select cell somata in the
inner nuclear layer (INL; arrowhead), in delicate puncta in the
inner plexiform layer (IPL), and in numerous somata in the
ganglion cell layer (GCL; vertical arrow). B: RAMP2-LI
was localized near the photoreceptor outer segments (diagonal
arrow), in somata in the ONL (horizontal arrow), and in somata
in the INL (arrowhead) and GCL (vertical arrow). Scale bars=20
µm. C: western blot of mouse retinal homogenate probed
with the same CRLR and RAMP2 antisera used for
immunocytochemistry. Both antisera recognized single proteins
with the correct molecular weights.