Figure 3 of Blom, Mol Vis 2012; 18:1339-1353.


Figure 3. Colocalization of adrenomedullin (ADM) with cell specific markers was done to accurately identify cell types with ADM-like immunoreactivity (LI). In all figures, ADM-LI was localized near the photoreceptor outer segments and in somata in the inner nuclear layer (INL) and the ganglion cell layer (GCL). A: Glutamine synthetase (GS, red) labeled Müller cells, and colocalized with ADM (green) in their somata in the INL (horizontal arrow). B: Protein kinase C α-like immunoreactivity (PKCα-LI; green) was present in rod bipolar cells and did not colocalize with ADM-LI (red). C: Calbindin-LI (green) was localized in horizontal cells in the INL and their process in the OPL, as well as in somata in the INL and GCL. ADM-LI (red) colocalized with calbindin-LI in somata in the lower tier of the INL that borders the IPL (vertical arrow) and in somata in the GCL (diagonal arrow). D: Calretinin-LI (green) was localized in amacrine cell somata in the INL, in processes in the inner plexiform layer (IPL), and within somata in the GCL. ADM-LI (red) did not colocalize with calretinin-LI. E: neuronal nitric oxide synthase (nNOS)-LI (green) was localized in select amacrine cell somata in the INL, in processes in the IPL, and in somata in the GCL. Some somata with nNOS-LI in the GCL colocalized with ADM-LI (red) (arrow). F: Glutamate-LI (red) was localized near the photoreceptor outer segments, in the OPL, in somata in the INL and the GCL, and as diffuse staining in the IPL. ADM-LI (green) colocalized with glutamate near the outer segments (arrow). G: Glycine-LI (red) was localized in the INL, in the OPL and the IPL, and in somata in the INL and the GCL. Glycine-LI was not colocalized with ADM-LI (green) in any somata, but there was some potential colocalization in puncta in the IPL (arrow). H: gamma-aminobutric acid (GABA)-LI (red) was localized to somata in the ONL, processes in the OPL, somata in the INL, diffusely in the IPL, and somata in the GCL. ADM-LI (green) did not colocalize with GABA-LI. Scale bars=20 µm.