Figure 1. Adrenomedullin (ADM), calcitonin receptor like receptor (CRLR), and eceptor activity modifying protein (RAMPs) needed for ADM signaling are expressed in the retina. A: Reverse transcriptase (RT)–PCR using ADM specific primers [23] showed a single band at the expected size of approximately 225 bp. Lane 1=ADM RT–PCR; Lane 2=no template control; Lane 3=NEB 50 bp ladder (New England BioLabs, Ipswich, MA), darkest band=200 bp. B: RT–PCR using primers described by Uzan et al. [23] confirmed the expression of CRLR (Lane 1, approximately 700 bp), RAMP3 (Lane 2, approximately 250 bp), and RAMP2 (Lane 3, approximately 190 bp). Lane 4=NEB 100 bp ladder.