Figure 5. Sirtuin1 (SIRT1) induced vascular endothelial growth factor (VEGF) release. A: Evaluation of VEGF levels in the conditioned media after the exposure of choroidal endothelial cells with hypoxia with or without blocking SIRT1 activity (200 uM sirtinol; mean+SE; n=3; *p<0.05) using enzyme-linked immunosorbent assay (ELISA). The x-axis represents the experimental conditions and the y-axis represents the VEGF protein levels in pg/ml. B: Correlation of SIRT1 levels measured in the presence of escalating VEGF. The x-axis represents the observed mean VEGF protein levels in pg/mL and y-axis represents their respective SIRT1 levels.