Figure 3. Evaluation of sirtuin1 levels at the cellular level. Choroidal endothelial cells (CECs) were cultured in a 96-well microtiter plate and treated with hypoxia for 24 h. Sirtuin1 (SIRT1) levels were evaluated in hypoxic cells with or without SIRT1 activity being blocked using sirtinol (Mean+SE; n=3;*p<0.05). The x-axis represents the experimental conditions and the y-axis represents the SIRT1 levels in percentage (normalized against control).