Figure 3. Expression and quantification of selected proteins in 661W cells treated with caffeic acid phenethyl ester (CAPE) and H₂O₂. A: Expression and quantification of heme oxygenase 1 (HO-1), cyclooxygenase 2 (COX-2), and IκBα proteins in 661W cells was measured by western blot analysis. Proteins were extracted and subjected to western blotting with anti-HO-1, anti-COX-2, and anti-IκBα antibodies. Lane 1(NT): no treatment; lanes 2 and 3 (caffeic acid phenethyl ester [CAPE]): CAPE treated; lanes 4 and 5 (H₂O₂): H₂O₂ treated; lanes 6 and 7 (C+H): pretreated with CAPE, then with H₂O₂. B: Quantification of COX-2 and IκBα in 661W cells with western blotting. Quantification of COX-2 and IκBα was obtained with densitometric analysis, and normalized with β-actin. (n=3–6; *: p<0.05, by the Student t test).