Figure 10. Expression and quantification of selected proteins in the retina from rats fed for eight weeks with control and caffeic acid phenethyl ester (CAPE) diet. Retinas were harvested from CAPE- and control diet-fed rats maintained under cyclic dim or bright light exposure for eight weeks. A: Retinal whole cell proteins were extracted and subjected to western blotting with anti-LOX5, and anti-COX-2 antibodies, and the cytoplasmic and nuclear fraction separately with anti-NFκB antibodies. B: Quantification of LOX5, COX-2, and NFκB (n) expression in nuclei and NFκB (c) in cytosol from the retinas was obtained with densitometric analysis, and normalized by β-actin, (n=4). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).