Figure 10. Expression and
quantification of selected proteins in the retina from rats fed
for eight weeks with control and caffeic acid phenethyl ester
(CAPE) diet. Retinas were harvested from CAPE- and control
diet-fed rats maintained under cyclic dim or bright light
exposure for eight weeks. A: Retinal whole cell proteins
were extracted and subjected to western blotting with anti-LOX5,
and anti-COX-2 antibodies, and the cytoplasmic and nuclear
fraction separately with anti-NFκB antibodies. B:
Quantification of LOX5, COX-2, and NFκB (n) expression in nuclei
and NFκB (c) in cytosol from the retinas was obtained with
densitometric analysis, and normalized by β-actin, (n=4).
Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet
under cyclic dim light; Cont+BL: control diet under cyclic
bright light; CAPE+BL: CAPE diet under cyclic bright light. (*:
p<0.05, with the Student t test).