Figure 5. Differentiation of human limbal stromal cells. A: Adipogenic differentiation could be induced as examined by Oil Red-O staining (magnification 200×). B: Osteogenic differentiation (100×). C: Chondrogenic differentiation (50×) as examined by Alizarin Red and Alcian Blue staining; The results of RT–PCR for the relevant transcripts after differentiation were also depicted (left lanes: uninduced cells; right lanes: induced cells).