Figure 4. Immunofluorescence staining of limbal stromal cells. The limbal stromal cells were positive for vimentin (200×; green), but dim positive for SSEA-4 (100×; green). Expression for ABCG2 (100×), p63 (100×), Pax 6 (100×), AE5 (100×), CK 10 (200×), keratocan sulfate (200×), α-SMA (200×), and Oct 3/4 (100×) proteins was found negative. The nuclei were counterstained either with propidium iodide (red) or DAPI (blue).