Figure 4. Transcription analysis of the mutant CRYBA1/A3 gene. A: RT–PCR products separated on a 2% agarose gel. CRYBA1-wt (RT–PCR products from the 293T cells transfected with pc3.1-CYRBA1-wt) showed a minor band, about 260 bp; CRYBA1-mt (RT–PCR products from the 293Tells transfected with pc3.1-CYRBA1-mt) showed a major band, about 1,992 bp. B: Graphic presentation of wild and mutant transcript, with the wild type indicating that exon 3-exon 4 were combined, except for intron 3, and the mutant transcript indicating that exon 3-intron 3-exon 4 were combined. C: Flank sequence of splice mutation. The red font indicates mutant base, the additional 24 amino acid residues are marked by underline and the stop coden is marked by black frame.