Figure 9. Rod outer segment phagocytosis by microglial cells. A: Confocal micrographs of microglial cells incubated with FITC-modified HNE-ROSs at 1 h (top, second) and at 24 h (top, third), and bleached-ROSs at 24 h (top, right). FITC-ROSs co-localized well with microglial cells that stained with a fluorescent acidotropic probe of Lysotracker Red, which show as yellow spots in pictures. There was more co-localization in HNE-ROSs than bleached-ROSs at 24 h. B: Color channel separation for the cells fed with HNE-modified ROSs at 24 h. Scale bar=10 μm.