Figure 2. Autofluorescence of macrophages and microglial cells after 4 day feeding with different rod outer segments (ROSs). A: This is a fluorescence-activated cell sorting (FACS) analysis of the fluorescein isothiocyanate (FITC) channel (detection filter, 533/30 nm) of the microglial cells (left panel) and macrophage cells (right panel) fed with different ROSs. Note the large increase of AF in cells fed with modified rod outer segments (HNE-ROSs) compared to the other two groups. B: FACS analysis in the PE channel (detection filter, 585/40 nm) of the microglial cells (left panel) and macrophage cells (right panel) fed with different ROSs. Note the slight increase in the bleached ROS group and the decrease in HNE-ROSs compared to the corresponding AF registering at 533 nm. Abbreviation key: BL-ROSs=bleached rod outer segments; UB-ROSs=unbleached rod outer segments. Each bar reflects the average value obtained from nine samples. Asterisks indicate statistical significance (one sample t-test; ***=p<0.001).