Figure 1. Spectrum of rhodopsin in rod outer segments. The characteristic rhodopsin absorbance at 498 nm was used to quantitate pigment yields. Note the absorbance difference at that wavelength between bleached rod outer segments (ROSs; green trace) and unbleached ROSs (orange trace). The modified rod outer segments (HNE-ROSs) at 5 mM (red trace) showed decrease in absorbance compared to unbleached ROSs.